Howdy /fit/, as maybe our only poster using a bigger anti-aging stack than Dave Pascoe, I figured I'd drop new senolytic research that Bryan Johnson *might* read in a few years...>Molecular Mechanisms of Cellular Senescence and Targeted Intervention with Natural Bioactive Compounds>www.sciencedirect.com/science/article/pii/S2950306X26000518>Association between sirtuin 1 and markers of oxidative stress in master athletes>link.springer.com/article/10.1007/s00421-026-06369-5>Phytotherapeutic Targeting of Mitochondria in Alzheimer's Disease: A Narrative Review>www.sciencedirect.com/science/article/pii/S2667031326000904>Protocatechuic acid counteracts cisplatin-induced oxidative testicular injury in rats through modulation of redox homeostasis>link.springer.com/article/10.1186/s12906-026-05604-y>Imeglimin halts cyclophosphamide-induced ovarian injury: a dual modulation of TLR4/NF-κB/NLRP3-driven inflammation and AMPK/SIRT1/PGC-1α pathways>link.springer.com/article/10.1007/s00210-026-05885-7>Xinmailong Injection Attenuates Doxorubicin-Induced Chronic Heart Failure by Regulating the SIRT1/Caspase-3 Axis and Metabolic Reprogramming: An Integrative Network Pharmacology and Metabolomics Study>link.springer.com/article/10.1007/s11596-026-00238-0>Cardioprotective role of vincamine in cyclophosphamide-induced cardiotoxicity through modulation of AMPK/SIRT1, PI3K/Akt, and MAPK pathways>link.springer.com/article/10.1007/s00210-026-05875-9>Syringaresinol activates the skeletal muscle SIRT1–FNDC5/irisin axis to enhance weight management>link.springer.com/article/10.1007/s13205-026-05027-z>Chemical characterization and pharmacological mechanisms of Qizhi Yishen Capsule in early diabetic kidney disease>www.sciencedirect.com/science/article/abs/pii/S0944711326010275>Mitochondrial homeostasis in musculoskeletal diseases: From pathogenic mechanisms to precision therapies>www.sciencedirect.com/science/article/pii/S1043661826003567Anyone else on the molecule hunt?
what stack will have me youthmaxxing the zoomers? t. 35 year old unc
>>77533402fucking their 20 year old milfs in their tight virgin asshole
>>775334028 beers by 2 pm
Lurking for more anti-aging schitzos to post
>le heckin sciencedirectYou're just a pleb with no understanding of the actual science behind this popsci article
>>77533372This nigga be livin for 300 years lmao
>Timing-Dependent Clearance of p16-Positive Cells Mitigates Radiation-Induced Accelerated Aging>https://onlinelibrary.wiley.com/doi/full/10.1111/acel.70693
>>77533372Enough with the soience, OP.Just lay down some simple shit that I can implement.
>>77534391This stuff is a little bit rare, but it's on Alibaba at least...>Aging-modulatory effects of Pausinystalia macroceras (K. Schum.) Pierre polysaccharides are associated with DAF-16/FOXO and SKN-1/Nrf2 signaling in multiple aging models>https://link.springer.com/article/10.1007/s10522-026-10509-x
>>77534391>>77534425>give it to me straight OP>OP replies with inscrutable technobabble, schizo-tier trump image, and a link to some bullshit sound
>>77534493>anon can't find a literal African traditional medicine with a recent anti-aging proteomic study>on literal Alibaba>alibaba.com/showroom/pau-de-cabinda-bark-powder.html
I hate this nigga like you wouldn't believeAnything but a treatment with a significant effect sizet. pharmacology schizo
>>77533372this fukken thread holy shit
>>77534710>Anything but a treatment with a significant effect sizeLel what do you propose; I.V. schizo yarmulke factors until u troon in2 giant teratoma?Aging is not as reductionist as Elon Musk types would like to believe, and even one-shot gene therapies like the Follistatin overexpression Bryan Johnson got are a bit weaksauce from a global proteomics perspective.
>>77534760>Aging is not as reductionistWhere did I imply that it is?Anyways if you want to look at shit that actually works, you could look at FGF21 analogues, FOXO4-DRI, ecdysteroids, thymalin, 17α-estradiol, ITPP for starters
>>77533372Wow, this sounds annoying, gay, and retarded.
>>77535065>claims aging isn't reductionist>ALSO claims only like half a dozen kinds of molecules "actually works"I think all your molecules have decent enough evidence, and I'm on several of them, but individually and in combination they still address only a fraction of known senoprotein targets.17α-estradiol has me a little sketched out from how lesbianized Bryan Johnson looks, while both it and thymalin largely overlap with ecdysterone in beneficial downstream effects anyway, so I just do ecdy out of those three.Anti-aging research has advanced significantly further than you appear to be aware of, though, and the number of at least *vaguely* promising lead compounds easily numbers well into the thousands by now if you include in vitro studies....like picrel freakin' broccoli which contains sulforaphane; please upd8 the pharmacopeia of the rock you live under with that one at least.>>77535090>lol ya bro just rot in2 old age as nature intended>2026If you're a tren-head who gives yourself liver cancer, I hope you at least give us some good clinical trial data on RC chemos instead of just choosing MAID.
Still looking for that stronger ecdy. Other than that I don't have anything. Just taking the usual stuff bromelain, sulforaphane, etc. Might look into some TCM herbal formulas.
What is the clinical relevance of any of this? The answer is, probably nothing. Yet.
>>77535154>Still looking for that stronger ecdy.Calonysterone?>>77535159Biohackers are typically more interested in athletic performance enhancement rather than disease treatment, but the correlation between high SIRT1 levels and high detoxification enzyme levels in master's athletes in one of the OP studies is some kind of indirect evidence it's all really the same thing. Reversing atrial fibrillation and chemotherapy-associated cardiotoxicity will likely be some of the first clinical applications of senolytics.>>77535098Might have found a decent hit for last-line "PCT" when tren usage goes *drastically* wrong...>Therapeutic targeting of hepatocellular carcinoma cells with antrocinol, a novel, dual-specificity, small-molecule inhibitor of the KRAS and ERK oncogenic signaling pathways>https://pubmed.ncbi.nlm.nih.gov/36565974/
>>77533372no i just eat blueberries and a few other thingsI train balancing in the sense of 'death/aging starts with the legs/feet', like when a walking stick or walker is needed, its a lot of time to train the thing or not to have the faculty or not. As well as walking jogging
>>77535098>>ALSO claims only like half a dozen kinds of molecules "actually works"Sure, there's tons of stuff that works in vitro or has a teeny-tiny effect size in vivo. I would not consider those to "actually work">while both it and thymalin largely overlap with ecdysterone in beneficial downstream effects anyway, so I just do ecdy out of those three.No offense anon but you have just shown me you are retarded. The point of thymalin is to regrow the thymus and keep the immune system healthy and active through old age to decrease your chances of dying to infectious disease. If you can't see that and only care about the "downstream effects" then you are missing the forest for the trees.>and the number of at least *vaguely* promising lead compounds easily numbers well into the thousands by now if you include in vitro studies."vaguely promising" yeah exactly. Tons of shit works in vitro, and even in vivo in mice. It's a meme for a reason. Most of these don't have strong mechanistic evidence for good effects at higher doses.>sulforaphane>probably going to mention resveratrol and other flavonoidsSure, it's good in diet. But you can't make a big difference with it.>>77535289Calonysterone is intriguing but we need more data. Mechanistically I can't argue too much with it because it's an ecdy derivative but right now taking it just seems like a waste of money when you could just dose more ecdy.>some kind of indirect evidence it's all really the same thingYeah this has been known for a while. A lot of the stuff I've mentioned is also used as PEDs.
>>77535312>No offense anon but you have just shown me you are retarded. The point of thymalin is to regrow the thymus and keep the immune system healthy and active through old age to decrease your chances of dying to infectious disease. If you can't see that and only care about the "downstream effects" then you are missing the forest for the trees.No offense anon, but it's pretty retarded not even studying the effects of the bug roids you take to realize you're getting duped buying thymalin...>Bidirectional effect of ecdysterone on thymocyte volume regulation and proliferation>https://pubmed.ncbi.nlm.nih.gov/40543544/>prolonged exposure of thymocytes to 20HE increased their proliferative activity under primary culture conditions (by ∼67 % after 24 h) without detectable change in the VSOR/VRAC activity. Consistent with this result, at the whole organism level, administration of 20HE per os for 5 days strongly stimulated thymic growth (by ∼61 %) and up-regulated the CVR efficiency of the cells isolated from the 20HE-treated animals (parameter RVD increase by ∼12 %).
>Local Low‐Frequency Vibration Enhances Wound‐Healing and Modulates Cellular Senescence in Tissues and Exudates in a Hyperglycemic Rat Model>onlinelibrary.wiley.com/doi/abs/10.1111/1346-8138.70486>Senescence Biomarkers Independently Predict Disease-Related Mortality in Chronic Kidney Disease>researchsquare.com/article/rs-10539671/latest>Senomorphic miR-3155b suppresses the senescence-associated secretory phenotype in therapy-induced senescence>frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2026.1868137/full>Metabolically Active but Dysfunctional: The Impact of Senescent Cells and SASP>journals.sagepub.com/doi/abs/10.1177/15330338261481909>Exercise and p21 signaling in central nervous system aging and senescence: A context- and dose-dependent relationship>sciencedirect.com/science/article/abs/pii/S1568163726003077>High-Throughput Phenotypic Screening Identifies the Phytochemical Melissic Acid that Mitigates SHED Cellular Senescence via the ADCY5/cAMP/CREB Axis>frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2026.1908181/full>Roles of various types of ginseng in aging and age-associated phenotypes>openresearch.okstate.edu/entities/publication/b8053d1c-993a-4694-ab5b-b35c07223d53>PCC1 treatment reshapes ribosomal, lysosomal and membrane-lipid transcriptional programs in therapy-induced senescent human stromal cells>biorxiv.org/content/10.64898/2026.08.17.745159.abstract>The multifaceted roles of cellular senescence in liver pathophysiology: from tissue repair to tumor progression>sciencedirect.com/science/article/pii/S1044532326000400>Targeting Cellular Senescence in Kidney Aging with Traditional Chinese Medicine: Potential Senolytic and Senomorphic Strategies>sciencedirect.com/science/article/pii/S027092952600032X>Regulation of alanine glutamine asparagine catabolism is common to mouse models that extend lifespan>nature.com/articles/s41514-026-00505-3
>Crataegus Species as a Source of Geroprotective Phytochemicals: Biological Activities, Molecular Mechanisms and Therapeutic Perspectives>mdpi.com/2075-1729/16/9/1478>Nrf2–Keap1 Axis Modulation by Phytofabricated Metal Oxide Nanoparticles in Mesenchymal Stem Cell Lineage Bias>onlinelibrary.wiley.com/doi/abs/10.1002/jbt.71091>Anti-inflammatory phytochemicals from medicinal herbs based on senoinflammation schema for healthy aging>link.springer.com/article/10.1007/s12272-026-01632-x>Phytochemical Investigation of Origanum calcaratum Extracts and in vitro Study against Cellular Senescence>thieme-connect.com/products/ejournals/html/10.1055/a-2928-9370>Asiaticoside attenuates endothelial cells senescence in diabetic retinopathy via CCND1-mediated mitophagy through the PI3K/AKT/mTOR pathway>sciencedirect.com/science/article/pii/S0378874126011116>Discovery of a small peptide that increases yeast lifespan by enhancing the function of APCCdh1 in nondividing quiescent cells>academic.oup.com/genetics/advance-article-abstract/doi/10.1093/genetics/iyag249/8802419>Strain-Dependent Fermentation Enhances the Bioactivity of Gracilariopsis lemaneiformis Polysaccharide-Rich Fractions>mdpi.com/2304-8158/15/18/3272>Silibinin Ameliorated Lipid Accumulation Through Insulin/IGF-1 signaling Pathway in HGI C. elegans>sciencedirect.com/science/article/abs/pii/S0955286326002494>Cycloastragenol extends lifespan and enhances stress resistance in Caenorhabditis elegans by inhibiting IIS pathway and activating p38 MAPK-mediated innate immunity>sciencedirect.com/science/article/abs/pii/S0014299926008071>Antioxidant and anti-aging effects of polysaccharides extracted from Camellia oleifera branches>scijournals.onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.70953>Catalpol extends lifespan through sod-3-mediated mitophagy in Caenorhabditis elegans>sciencedirect.com/science/article/abs/pii/S0167494326002748
>>77533372You're going to die at 70
>>77535941>>https://pubmed.ncbi.nlm.nih.gov/40543544/I appreciate you fishing for the single study of weak mechanistic evidence it might do something. I'm not disputing that. But thymalin has a mountain of real evidence that isn't simply in vitro. If anything, your study shows my point that while ecdysterone has broadly beneficial "downstream effects," those effects are only actually tangible in SOME ways, not all. I was really hoping you linked a smoking gun paper though, I was happy to be wrong.>>77536477>>77536785Any reason to think these are going to be more effective in humans than the actual drugs with actual evidence that I mentioned? This is your problem.
>>77537073The study on ecdy you called "in vitro" was really ALSO in vivo FYI. And you can generally assume some new research chemical may work better than an existing prescription drug if it has a lower binding affinity on the same target protein(s), even from an animal study if the targeted protein networks are highly conserved (aka basically never in Alzheimer's and cancer).
>Parishin B, a phenolic constituent of Gastrodia elata Blume, extends lifespan and improves stress resistance in C. elegans>sciencedirect.com/science/article/abs/pii/S0378874126012456>Selenylated Physalis pubescens polysaccharide extends lifespan and activates Nrf2/HO-1-mediated antioxidant defense>sciencedirect.com/science/article/abs/pii/S0045206826011028>CCR2-high monocyte membrane-coated ginger-derived exosome-like nanoparticles attenuate spinal cord injury by inhibiting ferroptosis-mediated neuronal death and neuroinflammation via Nrf2/GPX4 axis>sciencedirect.com/science/article/abs/pii/S0944711326010494>γ-Glutamylcysteine alleviates hypertension and vascular remodeling in SHR by suppressing oxidative stress through Keap1 S-glutathionylation>tandfonline.com/doi/abs/10.1080/13510002.2026.2734676>Protective Effects of Total Alkaloids of Rhizoma Corydalis Against Neuronal Ferroptosis Through a PPARγ-Dependent Mechanism in Diabetes-Associated Cognitive Impairment>mdpi.com/1424-8247/19/10/1506>Astragaloside IV as a Multi-Target Neuroprotective Candidate in Ischemic Stroke and Cerebral Ischemia-Reperfusion Injury: Molecular Mechanisms and Therapeutic Perspectives>journals.sagepub.com/doi/abs/10.1177/1934578X261490290>Preparation and antioxidant activity of hydrolysates from Litsea cubeba oil processing residuals: fluorescence chromatography and molecular dynamics simulation>nature.com/articles/s41538-026-01171-1>Acteoside alleviates Parkinson's disease-associated neurodegeneration and oxidative stress via an m6A-dependent Nrf2 regulatory axis>sciencedirect.com/science/article/pii/S0753332226009819>Phytochemicals and bioactivities of Achillea alpina L.: bridging traditional knowledge to pharmacological evidence>link.springer.com/article/10.1007/s11101-026-10301-2>Repurposing Pimozide as a Geroprotector: Lifespan Extension via SKN-1 Activation and Collagen Remodeling in C. elegans>mdpi.com/2076-3921/15/9/1217
Taking some bioregulators
>>77536477>Regulation of alanine glutamine asparagine catabolism is common to mouse models that extend lifespan>nature.com/articles/s41514-026-00505-3Picrel has some reverse network pharmacology hits of phytochemicals with activity most similar to trametinib.
>Neuromuscular junction failure in sarcopenia is linked to NaV1.4 loss and reversed by ClC-1 inhibition>https://www.jci.org/articles/view/190646
>>77537430>ALSO in vivoDid you miss the fact that they used hundreds of μM (so close to mM really) concentrations of ecdy? I did read the entire abstract>if it has a lower binding affinityThis is sometimes true but you also have to consider competitive inhibition. IC50 is generally more predictive but even that doesn't account for heterodimers (usually rare unless you're looking at brain or super ubiquitous stuff) and cofactor/corepressor recruitment (very common especially with hormones)Which is to say that in vivo studies in humans remain the best way of actually knowing what a drug does. In vivo in rats is a good secondary but should not be trusted for less conserved systems (things that aren't muscle or metabolism really).>>77538015>>77538368>>77539382Care to explain what you should actionably do as a result of these papers instead of just posting links and looking smug?
>>77533372>a bigger anti-aging stack than Dave Pascoewhats the stack. what are the main important/easy components.>that Bryan Johnson *might* readso you're telling me that this isn't bryan johnsonhow old/young are you and are expecting to get to>TenPayGo Yāoguǐ Snek Out-Oiling Bread: Super-Strange Senolytics Editionmade me kekI butcher tea by mixxing matcha with raw cocoa and some coffeeI take essential greens power with OJ and think I'll add lemon and limeblueberries and sometimes other other berriesI make a soup with bryan johnson's vegetable stack and some other stuff. i eat some fermented stuff and hope it has some effect against microplasticall certified organic where I canMy hunch is that tea and spices have a key, sometimes herbs like oreganogarlic, honey, olive oil, chocolate, cinnamon
>>77540443the predictor of death is the loss of balance and loss of ambulatory ability (walking) I train balancing because its a giant oversight to lose the ability never training the thing over the whole lifespan its no wonder the stick and walker is neededthe real component of life is the mind's state
It's not difficult peopleDrink water, don't smoke, don't drink, have a skincare routine, and be mindful of the sun. Done.
>>77535098Post before and after results of whatever you've been taking. Face and body.
>>77540454not bad advice but obviously falls short. While I cant follow what op does I also think you are being overly simplistic in disregarding any potential for benefits.You fags either are science fags or dumbfags. Me as a smart fag but not a science fag can only keep to my personal stack and habits and maybe google some shi the science fags discuss.
Meanwhile I bet some of you guys disregard blood sugar levels as one important causal factor in longevity. Just eat salad before you eat carbs and stuff. Also no eating 4 hours before bed. When cooking add a little bit of citrus juice this makes it healthier. What about breath exercises and cold showers? Obviously do normal exercises but these are also beneficial.
Hey OP, you've read a gorillion PubMed articles and you've taken over 9000 anti aging compounds. Let's see the results. Post your age and a time stamped photo so we can see how much younger than your chronological age you look.
Cooking with a bit of citrus juice reduces AGEs. That was it.
AI: Cooking with high, dry heat forms advanced glycation end products (AGEs) when sugars and proteins bind together.What is Glycation in Cooking?The Process: Glycation in food happens through the Maillard reaction—a non-enzymatic reaction between reducing sugars and amino acids/proteins when exposed to heat.The Result: It creates dietary AGEs (dAGEs) like carboxymethyl lysine (CML), which give food a brown color and rich flavor (like a steak crust or toasted bread).Health Impact: Consuming high amounts of dietary AGEs can contribute to oxidative stress, inflammation, and cellular damage in the body.Cooking Methods to Reduce AGEsLow-Heat & Moist Methods: Boiling, steaming, poaching, and stewing use water and lower temperatures, which heavily limit AGE formation.High-Heat & Dry Methods: Frying, grilling, roasting, and toasting produce much higher levels of AGEs due to intense, dry heat.Practical Tips to Lower AGE FormationUse Acid: Marinating meats in acidic ingredients like lemon juice, lime juice, or vinegar for an hour before cooking can cut AGE formation by up to half.Add Moisture: Cook with liquids or use a pressure cooker/Instant Pot instead of open-flame charring.Avoid Charring: Do not blacken or overcook meats and vegetables; cut away charred, blackened edges.Use Antioxidants: Cooking with herbs and spices rich in antioxidants (like garlic, rosemary, and turmeric) helps counteract AGE creation.
>>77540471And a healthy dietAfter that it's diminishing returns
>>77540482>Also no eating 4 hours before bedYes a GI specialist once told me that >What about breath exercises and cold showers Obviously do normal exercises but these are also beneficialI start with warm (never hot) water and end with cold water for a minute.
>Epimedin B ameliorates benzo [a] pyrene-induced C2C12 myotube atrophy through p53 signaling pathway>https://www.sciencedirect.com/science/article/pii/S0898656826005656>NooQ5 Supplementation Promotes Myogenic Differentiation in C2C12 Cells Under Chemical and Oxidative Stress Models>https://www.mdpi.com/2073-4409/15/19/1719>β‐Nicotinamide Mononucleotide Inhibits Cellular Senescence in Mouse C2C12 Skeletal Muscle Cells>https://pmc.ncbi.nlm.nih.gov/articles/PMC13598431/>Allium tuberosum extract attenuates dexamethasone-induced muscle atrophy by modulating the PI3K/AKT pathway>https://www.sciencedirect.com/science/article/abs/pii/S2212429226017529
>>77540650Thats just not true. You like to have it simple because some complexity is scary but live aint always simple.
>Dual-Molecule Senolytic Strategy Constructed by Senescence-Associated β-galactosidase-Activated Tetrazine Tagging and IEDDA-Triggered Gemcitabine Release>https://www.sciencedirect.com/science/article/abs/pii/S0143720826006972
>schizobabble and article posting continues >not interacting with the rest of the posters >frog collection either this is a low-functioning autist living in a pigsty somewhere or a broken machine
my stack:quercetinefisetineresveratrolpeppernmngreen teavitamin d3, k2magnesiumcinnamoncoffeeberberineZincnacashwagandaprobiotics
>>77543001Very legit normie stack with a lot of good evidence, but would benefit from several adjuvant additions including; glycine, copper, and boron for the NAC. The evidence for good interventions stretches far further however, pic definitely not related.
Good thread keep it up, saving me a lot of searching and also giving me some fun stuff to look into
>>77540486He always has the same excuse “No I won’t post body. It’s gay”Lmao
>>77543001Unironically better stack than the thread schizo because it's full of shit that actually works, even if it's not optimal
>>77543174>>77543732On a subjective and experiental level Im doing well on that stack. Other things not many people know:You can somewhat reverse myopia. There is a huge correlation between close-up work and myopia, it is even a causal link. If you scale your glasses you can gradually lessen or completely reverse myopia in most but not all cases.Longevity is not everything and the world is big
