>be me>PhD student>have cells>have reagents>have antibodies>have everything ready>need one fucking chamber slide>Nunc™ Lab-Tek™ II CC2™>Thermo Fisher says no>entire scientific career collapses>can't do experiment>can't collect data>can't graduate>my thesis is now a missing persons report for a fucking chamber slide>professor asks when the results will be readyWHEN THE FUCKING GLASS ARRIVES
Haven't you heard? The world's economy is collapsing.
>>17088138ANONI DON'T GIVE A FUCK ABOUT THE GLOBAL ECONOMYTHE WORLD CAN ENTER A RECESSIONTHE STOCK MARKET CAN GO TO ZEROTHE IMF CAN DECLARE BANKRUPTCYBUT MY FUCKING CELLS HAVE AN EXPERIMENT SCHEDULETHEY DID NOT SIGN UP FOR QUANTITATIVE EASINGTHEY SIGNED UP FOR IMMUNOFLUORESCENCETHERMO FISHER HAS ONE JOBSHIP THE FUCKING GLASS
Sounds like your ass is glass
>>17088136Just put wells and a coating on a normal slide. Order some stuff off of Amazon and fucking figure it out anon, that's your job isn't it?
>>17088136Could you give us more details on your experiment, anon? You can etch/coat polylysine slides yourself, but it was a bitch to get it playing nice with anything but the old confocal.Alternatively, PIs tend to have tons of odd shit lying around from old experiments, one of them might be willing to help out if you ask around
you sound like a pussy that's why you always keep trash slides and store them at home until you need themI did thermo-fisher waste pickups every week and my garage is full of their shit that I part out. I make an extra $2-3k per month this way. I do it as part of my dad's pick-n-pull puller business.
>>17088140you can just make a new one with a glass cutter and a broken window from someone's trashor a car window from a salvage lot if you are feeling fancyI remember doing this in highschool when I cheated on my chemistry final, which was the same thing but with spider eggs that hatched in my fridge
>>17088136>>17088138Is that why 12 ft. of red rubber tubing is $1000?
>>17088582lol what the actual fuck?
>>17088136damn thats a ripoff why is it so expansive?
>>17088582>>17088703Because there's only one company that sells it, the people buying it aren't paying for it with their own money, and the organisation that gives them money gets budgeted more money the more they spend.
>>17088140>>17088153>OP anon is freaking out and helpful anon arrives with sage advice. OP anon disappears however. this kind of thing is too common here. Does /sci/ drive OP anons to suicide so rapidly that they can’t wait to talk to the helpful anons and an hero within like an hour? is it so over?I get the feeling that a lot of threads here have this weird tendency that once a knowledgeable anon shows up and shells out some wisdom (for free btw, unlike other platforms where everyone is making profits off engagement) then the thread dies. Instead the threads that get 300 replies are idiot echo chambers or shill containment threads. Like nobody is here to appreciate the smart anons who hand out advice for free since we’re just autistic like that. Why is that? You’d figure at least a few newfags would want to take advantage of our free advising services, but lo and behold they leave free services on the table. I guess the internet has become a “low-trust society” or something where the only acceptable form of engagement is being adversarial and hateful
>>17088136>be pHD student>be 100% aware you have an upcoming thesis to present>wait until the last minute to order parts that are critical to your success>order is delayed>REEEE!!!! THE COMPANY DID THIS TO ME!Crazy how even PHDs can completely lack common sense. You brought this upon yourself and then immediately blamed an outside source. You are pathetic, OP.
>>17088582>12ft38mm is 0.12ft
>>17088136>WHEN THE FUCKING GLASS ARRIVESgo back, please
>>17088724Not in opposite language.
>>17088731>posting some obscure racism wojak memego back please
>>17088741it'sWHEN DOES THE FUCKING GLASS ARRIVE?or WHEN ARRIVES THE FUCKING GLASS? (if you wish to be archaic)please learn english before coming here and by here i mean 4chan
>>17088582>Is that why 12 ft. of red rubber tubing is $1000?hope it's bait, because 38/19mm! diameter! length unknown and yes you are at le worst site for science where stupidity meet ignorance.
>>17088716Lmao fuck you anon. You think I'm going to leak my university's research secrets for free? This isn't an NIH grant application, you fucking intellectual vulture. The university didn't spend years training me just for some random /sci/ goblin to harvest my research like free-range data. Nice try. Classified by the Department of Gatekeeping.
>>17088136You should've arranged the order in advance, anon, and beyond that - betting it all on one hyper-specific proprietary consumable is a poor call. I always teach my grad students that a well conducted experiment on more primitive gear beats the shiniest toys for a dissertation work. It serves better to show a good grasp of fundamentals, while the top-line gear only serves to burn funds, create bottlenecks and antagonize the committee dinosaurs that will do the evaluation - they think shiny stuff does all the Real Work(tm) for you at best, and at worst their lack of expertise with it makes them feel inadequate.But hey, at least you learned not to put your trust into Thermo Fisher Scientific Inc.
>>17089330>PhD student>research secretsBruh you do remember that your dissertation will be publicly accessible, right?
>>17089341I am not an expert in this field, but in my field (experimental physics) I would also have to agree that this is a problem. You can’t do your measurements without the special Techtronix scope? You need to buy the special CAEN analog-to-digital converter? You need so many DSPs that only the latest Xilinx FPGA can do it? usually this means you’ve been grifted by some salesman and if you did your homework you could figure a DIY solution with decade-old tech. Plus using the old tech and the old methods means all the hardware-related issues are already solved (you probably won’t need to debug their proprietary firmware through their user forum, which is all to common when you buy a shiny new box). If you find yourself pigeonholed into buying some shiny new gadget then it is a symptom of doing things wrong
>>17088136>>Thermo Fisher says nowhat do you mean they said no lmao it seems that they have it in stockthat said ask whether somebody else in your institute have them or just find an alternative from another vendor. I don't think cells with poor adhesion can be cultured only in these chambers. Check your literature.That said AI sloppa for you: >Corning 354577 is the closest functional replacement; ibidi 80424 is the strongest alternative to investigate for continued procurement. Neither should be assumed to reproduce CC2 performance without experimental validation
>>17088136this is when you raid other lab groups, use the network
>>17088760Re-read OP and realise that line is a response to the bottom line of the greentext
>>17088136>go to grad school>realize you can't plan effectively>crash and burn and blame it on someone elseso sad, many such cases
>>17088724That's the outer diameter no tthe length, which is given at the bottom of the page under Specifications.
>>17089310>length unknownIt's 12 ft. I assure you.
>>17088136The entire global supply chain is collapsing.That's why I've always said everything should be produced nationally (US, Europe, etc.).
